Inbred corn line G07-NPID5459

ABSTRACT

Basically, this invention provides for an inbred corn line designated G07-NPID5459, methods for producing a corn plant by crossing plants of the inbred line G07-NPID5459 with plants of another corn plant. The invention relates to the various parts of inbred G07-NPID5459 including culturable cells. This invention also relates to methods for introducing transgenic transgenes into inbred corn line G07-NPID5459 and plants produced by said methods.

FIELD OF THE INVENTION

This invention is in the field of corn breeding, specifically relatingto an inbred corn line designated G07-NPID5459. This invention also isin the field of hybrid maize production employing the present inbred.

BACKGROUND OF THE INVENTION

The original maize plant was indigenous to the Western Hemisphere. Theplants were weed like and only through the efforts of early breederswere cultivated crop species developed. The crop cultivated by earlybreeders, like the crop today, could be wind pollinated. The physicaltraits of maize are such that wind pollination results inself-pollination or cross-pollination between plants. Each maize planthas a separate male and female flower that contributes to pollination,the tassel and ear, respectively. Natural pollination occurs when windtransfers pollen from tassel to the silks on the corn ears. This type ofpollination has contributed to the wide variation of maize varietiespresent in the Western Hemisphere.

The development of a planned breeding program for maize only occurred inthe last century. A large part of the development of the maize productinto a profitable agricultural crop was due to the work done by landgrant colleges. Originally, maize was an open pollinated variety havingheterogeneous genotypes. The maize farmer selected uniform ears from theyield of these genotypes and preserved them for planting the nextseason. The result was a field of maize plants that were segregating fora variety of traits. This type of maize selection led to; at most,incremental increases in seed yield.

Large increases in seed yield were due to the work done by land grantcolleges that resulted in the development of numerous hybrid cornvarieties in planned breeding programs. Hybrids were developed frominbreds which were developed by selecting corn lines and selfing theselines for several generations to develop homozygous pure inbred lines.One selected inbred line was emasculated and another selected inbredline pollinated the emasculated inbred to produce hybrid seed F1 on theemasculated inbred line. Emasculation of the inbred usually is done bydetasseling the seed parent; however, emasculation can be done in anumber of ways. For example an inbred could have a male sterility factorwhich would eliminate the need to detassel the inbred.

In the early seventies the hybrid corn industry attempted to introduceCMS (cytoplasmic male sterility) into a number of inbred lines.Unfortunately, the CMS inbreds also introduced some very poor agronomicperformance traits into the hybrid seed which caused farmers concerncausing the maize industry to shy away from CMS material for a couple ofdecades thereafter.

However, in the last 10-15 years a number of different male sterilitysystems for maize have been successfully deployed. The mosttraditionally of these male sterility and/or CMS systems for maizeparallel the CMS type systems that have been routinely used in hybridproduction in sunflower.

In the standard CMS system there are three different maize linesrequired to make the hybrid. First, there is a cytoplasmic male-sterileline usually carrying the CMS or some other form of male sterility. Thisline will be the seed producing parent line. Second, there must be afertile inbred line that is the same or isogenic with the seed producinginbred parent but lacking the trait of male sterility. This is amaintainer line needed to make new inbred seed of the seed producingmale sterile parent. Third there is a different inbred which is fertile,has normal cytoplasm and carries a fertility restoring gene. This lineis called the restorer line in the CMS system. The CMS cytoplasm isinherited from the maternal parent (or the seed producing plant);therefore for the hybrid seed produced on such plant to be fertile thepollen used to fertilize this plant must carry the restorer gene. Thepositive aspect of this is that it allows hybrid seed to be producedwithout the need for detasseling the seed parent. However, this systemdoes require breeding of all three types of lines: 1) male sterile-tocarry the CMS; 2) the maintainer line; and, 3) the line carrying thefertility restorer gene.

In some instances, sterile hybrids are produced and the pollen necessaryfor the formation of grain on these hybrids is supplied by interplantingof fertile inbreds in the field with the sterile hybrids.

Whether the seed producing plant is emasculated due to detasseling orCMS or transgenes, the seed produced by crossing two inbreds in thismanner is hybrid seed. This hybrid seed is F1 hybrid seed. The grainproduced by a plant grown from a F1 hybrid seed is referred to as F2 orgrain. Although, all F1 seed and plants, produced by this hybrid seedproduction system using the same two inbreds should be substantially thesame, all F2 grain produced from the F1 plant will be segregating maizematerial.

The hybrid seed production produces hybrid seed which is heterozygous.The heterozygosis results in hybrid plants, which are robust andvigorous plants. Inbreds on the other hand are mostly homozygous. Thishomozygosity renders the inbred lines less vigorous. Inbred seed can bedifficult to produce since the inbreeding process in corn linesdecreases the vigor. However, when two inbred lines are crossed, thehybrid plant evidences greatly increased vigor and seed yield comparedto open pollinated, segregating maize plants. An important consequenceof the homozygosity and the homogenity of the inbred maize lines is thatall hybrid seed produced from any cross of two such elite lines will bethe same hybrid seed and make the same hybrid plant. Thus the use ofinbreds makes hybrid seed which can be reproduced readily.

The ultimate objective of the commercial maize seed companies is toproduce high yielding, agronomically sound plants that perform well incertain regions or areas of the Corn Belt. To produce these types ofhybrids, the companies must develop inbreds, which carry needed traitsinto the hybrid combination. Hybrids are not often uniformly adapted forthe entire Corn Belt, but most often are specifically adapted forregions of the Corn Belt. Northern regions of the Corn Belt requireshorter season hybrids than do southern regions of the Corn Belt.Hybrids that grow well in Colorado and Nebraska soils may not flourishin richer Illinois and Iowa soils. Thus, a variety of major agronomictraits is important in hybrid combination for the various Corn Beltregions, and has an impact on hybrid performance.

Inbred line development and hybrid testing have been emphasized in thepast half-century in commercial maize production as a means to increasehybrid performance. Inbred development is usually done by pedigreeselection. Pedigree selection can be selection in an F2 populationproduced from a planned cross of two genotypes (often elite inbredlines), or selection of progeny of synthetic varieties, open pollinated,composite, or backcrossed populations. This type of selection iseffective for highly inheritable traits, but other traits, for example,yield requires replicated test crosses at a variety of stages foraccurate selection.

Maize breeders select for a variety of traits in inbreds that impacthybrid performance along with selecting for acceptable parental traits.Such traits include: yield potential in hybrid combination; dry down;maturity; grain moisture at harvest; greensnap; resistance to rootlodging; resistance to stalk lodging; grain quality; disease and insectresistance; ear and plant height. Additionally, hybrid performance willdiffer in different soil types such as low levels of organic matter,clay, sand, black, high pH, low pH; or in different environments such aswet environments, drought environments, and no tillage conditions. Thesetraits appear to be governed by a complex genetic system that makesselection and breeding of an inbred line extremely difficult. Even if aninbred in hybrid combination has excellent yield (a desiredcharacteristic), it may not be useful because it fails to haveacceptable parental traits such as seed yield, seed size, pollenproduction, good silks, plant height, etc.

To illustrate the difficulty of breeding and developing inbred lines,the following example is given. Two inbreds compared for similarity of29 traits differed significantly for 18 traits between the two lines. If18 simply inherited single gene traits were polymorphic with genefrequencies of 0.5 in the parental lines, and assuming independentsegregation (as would essentially be the case if each trait resided on adifferent chromosome arm), then the specific combination of these traitsas embodied in an inbred would only be expected to become fixed at arate of one in 262,144 possible homozygous genetic combinations.Selection of the specific inbred combination is also influenced by thespecific selection environment on many of these 18 traits which makesthe probability of obtaining this one inbred even more remote. Inaddition, most traits in the corn genome are regrettably not singledominant genes but are multi-genetic with additive gene action notdominant gene action. Thus, the general procedure of producing a nonsegregating F1 generation and self pollinating to produce a F2generation that segregates for traits and selecting progeny with thevisual traits desired does not easily lead to an useful inbred. Greatcare and breeder expertise must be used in selection of breedingmaterial to continue to increase yield and the agronomics of inbreds andresultant commercial hybrids.

Certain regions of the Corn Belt have specific difficulties that otherregions may not have. Thus the hybrids developed from the inbreds haveto have traits that overcome or at least minimize these regional growingproblems. Examples of these problems include in the eastern Corn BeltGray Leaf Spot, in the north cool temperatures during seedlingemergence, in the Nebraska region CLN (Corn Lethal Necrosis) and in thewest soil that has excessively high pH levels. The industry oftentargets inbreds that address these issues specifically forming nicheproducts. However, the aim of most large seed producers is to provide anumber of traits to each inbred so that the corresponding hybrid can beuseful in broader regions of the Corn Belt. The new biotechnologytechniques such as Microsatellites, RFLPs, RAPDs and the like haveprovided breeders with additional tools to accomplish these goals.

SUMMARY OF THE INVENTION

The present invention relates to an inbred corn line G07-NPID5459.Specifically, this invention relates to plants and seeds of this line.Additionally, this relates to a method of producing from this inbred,hybrid seed corn and hybrid plants with seeds from such hybrid seed.More particularly, this invention relates to the unique combination oftraits that combine in corn line G07-NPID5459.

Generally then, broadly the present invention includes an inbred cornseed designated G07-NPID5459. This seed produces a corn plant.

The invention also includes the tissue culture of regenerable cells ofG07-NPID5459 wherein the cells of the tissue culture regenerates plantscapable of expressing the genotype of G07-NPID5459. The tissue cultureis selected from the group consisting of leaf, pollen, embryo, root,root tip, guard cell, ovule, seed, anther, silk, flower, kernel, ear,cob, husk and stalk, cell and protoplast thereof. The corn plantregenerated from G07-NPID5459 or any part thereof is included in thepresent invention. The present invention includes regenerated cornplants that are capable of expressing G07-NPID5459's genotype, phenotypeor mutants or variants thereof.

The invention extends to hybrid seed produced by planting, inpollinating proximity which includes using preserved maize pollen asexplained in U.S. Pat. No. 5,596,838 to Greaves, seeds of corn inbredlines G07-NPID5459 and another inbred line if preserved pollen is notused; cultivating corn plants resulting from said planting; preventingpollen production by the plants of one of the inbred lines if two areemployed; allowing cross pollination to occur between said inbred lines;and harvesting seeds produced on plants of the selected inbred. Thehybrid seed produced by hybrid combination of plants of inbred corn seeddesignated G07-NPID5459 and plants of another inbred line are apart ofthe present invention. This inventions scope covers hybrid plants andthe plant parts including the grain and pollen grown from this hybridseed.

The invention further includes a method of hybrid F1 production. A firstgeneration (F1) hybrid corn plant produced by the process of plantingseeds of corn inbred line G07-NPID5459; cultivating corn plantsresulting from said planting; permitting pollen from another inbred lineto cross pollinate inbred line G07-NPID5459; harvesting seeds producedon plants of the inbred; and growing a harvested seed are part of themethod of this invention.

The present invention also encompasses a method of introducing at leastone targeted trait into maize inbred line comprising the steps of: (a)crossing plant grown from the present invention seed which is therecurrent parent, representative seed of which has been deposited, withthe donor plant of another maize line that comprises at least one targettrait selected from the group consisting of male sterility, herbicideresistance, insect resistance, disease resistance, amylose starch, andwaxy starch to produce F1 plants; (b) selecting from the F1 plants thathave at least one of the targeted traits, forming a pool of progenyplants with the targeted trait; (c) crossing the pool of progeny plantswith the present invention which is the recurrent parent to producebackcrossed progeny plants with the targeted trait; (d) selecting forbackcrossed progeny plants that have at least one of the target traitsand physiological and morphological characteristics of maize inbred lineof the recurrent parent, listed in Table 1 forming a pool of selectedbackcrossed progeny plants; and (e) crossing the selected backcrossedprogeny plants to the recurrent parent and selecting from the resultingplants for the targeted trait and physiological and morphologicalcharacteristics of maize inbred line of the recurrent parent, listed inTable 1 and reselecting from the pool of resulting plants and repeatingthe crossing to the recurrent parent and selecting step in succession toform a plant that comprise the desired trait and all of thephysiological and morphological characteristics of maize inbred line ofthe recurrent parent if the present invention listed in Table 1 asdetermined at the 5% significance level when grown in the sameenvironmental conditions.

This method and the following method of introducing traits can be donewith less backcrossing events if the trait and/or the genotype of thepresent invention are selected for or identified through the use ofmarkers. SSR, microsatellites, SNP and the like decrease the amount ofbreeding time required to locate a line with the desired trait or traitsand the characteristics of the present invention. Backcrossing in two oreven three traits (for example the glyphosate, Europe Corn Borer, CornRootworm resistant genes) is routinely done with the use of markerassisted breeding techniques. This introduction of transgenes ormutations into a maize line is often called single gene conversion.Although, presently more than one gene particularly transgenes ormutations which are readily tracked with markers can be moved during thesame “single gene conversion” process, resulting in a line with theaddition of more targeted traits than just the one, but still having thecharacteristics of the present invention plus those characteristicsadded by the targeted traits.

The method of introducing a desired trait into maize inbred linecomprising: (a) crossing a plant grown from the seed of the presentinvention, (representative seed on deposit) this plant being therecurrent parent, with at least one plant of another maize line thatcomprises at least one target trait selected from the group consistingof nucleic acid encoding an enzyme selected from the group consisting ofphytase, stearyl-ACP desaturase, fructosyltransferase, levansucrase,amylase, invertase and starch branching enzyme, starch synthase,debranching enzyme, this plant being the donor parent to produce F1plants; (b) selecting for at least one of the targeted trait from the F1plants, (c) forming a pool of progeny plants with the target trait; (d)crossing the progeny plants with the recurrent parent to producebackcrossed progeny plants; (e) selecting from the backcrossed progenyplants that have at least one of the target traits and the physiologicaland morphological characteristics of maize inbred line of the presentinvention f) forming a new pool of backcrossed progeny plants; andrepeating the step of crossing the new pool of backcrossed progencyplant with the recurrent parent and selecting for the targeted trait andthe recurrent parent's characteristics until the selected plant isessentially the recurrent parent with the targeted trait or targetedtraits. This selection and crossing may take 1, 2, 3, 4, 5, 6 or morebackcrosses. Marker assisted breeding may limit the need for numerousbackcrosses.

The inbred line and seed of the present invention are employed to carrythe agronomic package into the hybrid. Additionally, the inbred line isoften carrying transgenes that are introduced in to the hybrid seed.

Likewise included is a first generation (F1) hybrid corn plant producedby the process of planting seeds of corn inbred line G07-NPID5459;cultivating corn plants resulting from said planting; permitting pollenfrom inbred line G07-NPID5459 to cross pollinate another inbred line;harvesting seeds produced on plants of the inbred; and growing a plantfrom such a harvested seed.

A number of different techniques exist which are designed to avoiddetasseling in maize hybrid production. Some examples are switchablemale sterility, lethal genes in the pollen or anther, inducible malesterility, male sterility genes with chemical restorers. There arenumerous patented means of improving upon the hybrid production system.Some examples include U.S. Pat. No. 6,025,546, which relates to the useof tapetum-specific promoters and the barnase gene to produce malesterility; U.S. Pat. No. 6,627,799 relates to modifying stamen cells toprovide male sterility. Therefore, one aspect of the current inventionconcerns the present invention comprising one or more gene(s) capable ofrestoring male fertility to male-sterile maize inbreds or hybrids and/orgenes or traits to produce male sterility in maize inbreds or hybrids.

The inbred corn line G07-NPID5459 and at least one transgene adapted togive G07-NPID5459 additional and/or altered phenotypic traits are withinthe scope of the invention. Such transgenes are usually associated withregulatory elements (promoters, enhancers, terminators and the like).Presently, transgenes provide the invention with traits such as insectresistance, herbicide resistance, disease resistance, increased ordeceased starch or sugar or oil, increased or decreased life cycle orother altered traits.

The present invention includes inbred corn line G07-NPID5459 and atleast one transgene adapted to give G07-NPID5459 modified starch traits.Furthermore this invention includes the inbred corn line G07-NPID5459and at least one mutant gene adapted to give modified starch, acid oroil traits, i.e. waxy, amylose extender or amylose desaturase and mutantgenes encoding starch synthase, starch branching and starch debranchingenzymes and amylase. The present invention includes the inbred corn lineG07-NPID5459 and at least one transgene: a Bt (bacillus thuringiensis),Cry or VIP gene, the bar or pat gene encoding Phosphinothricin acetylTransferase, gdhA, GOX, VIP (vegative insecticidal protein), EPSPencoding gene, low phytic acid producing gene, and zein. The inbred cornline G07-NPID5459 and at least one transgene useful as a selectablemarker or a screenable marker is covered by the present invention.

A tissue culture of the regenerable cells of hybrid plants produced withuse of G07-NPID5459 genetic material is covered by this invention. Atissue culture of the regenerable cells of the corn plant produced bythe method described above is also included.

DEFINITIONS

In the description and examples, which follow, a number of terms areused. In order to provide a clear and consistent understanding of thespecifications and claims, including the scope to be given such terms,the following definitions are provided.

Early Season Trait Codes

Emergence (EMRGR) or (Emerge): Recorded when 50% of the plots in thetrial are at V1 (1 leaf collar) growth stage.

1=All plants have emerged and are uniform in size

3=All plants have emerged but are not completely uniform

5=Most plants have emerged with some just beginning to break the soilsurface, noticeable lack of uniformity

7=Less than 50% of the plants have emerged, and lack of uniformity isvery noticeable

9=A few plants have emerged but most remain under the soil surface.

Seedling Growth (SVGRR) or (Vigor): Recorded between V3 and V5 (3-5 leafstage) giving greatest weight to seedling plant size and secondaryweight to uniform growth.

1=Large plant size and uniform growth

3=Acceptable plant size and uniform growth

5=Acceptable plant size and might be a little non-uniform

7=Weak looking plants and non-uniform growth

9=Small plants with poor uniformity

Purpling (PRPLR): Emergence and/or early growth rating. Purpling is morepronounced on the under sides of leaf blades especially on midribs.

1=No plants showing purple color

3=30% plants showing purple color

5=50% plants showing purple color

7=70% plants showing purple color

9=90+% plants showing purple color

Herbicide Injury (HRBDR) List the herbicide type, which is being rated.Then rated each hybrid/variety injury as indicated below.

1=No apparent reduction in biomass or other injury symptoms

5=Moderate reduction in biomass with some signs of sensitivity

9=Severe reduction in biomass with some mortality

Mid-Season Traits Codes

Heat Units to 50% Silk (HU5SN) or (S50): Recorded the day when 50% ofall plants within a plot show 2 cm or more silk protruding from the ear.Converted days to accumulated heat units from planting.

Heat units to 50% Pollen Shed (HUPSN) or (P50): Recorded the day when50% of all plants within a plot are shedding pollen. Converted days toaccumulated heat units from planting.

Plant Height in cm (ERHTN) or (Pltht): After pollination, recordedaverage plant height of each plot. Measured from ground to base of leafnode. Three or more locations recorded.

Root Lodging Early % (ERTLP): Early root lodging occurs up to about twoweeks after flowering and usually involves goosenecking. Counted thenumber of root lodged plants and converted to percentage. For FieldEvaluation Test plots (FET), recorded lodged plants out of 50 plantsfrom two locations in each hybrid strip, sum, and record percentage.Foliar Disease (LFDSR): Foliar disease ratings taken one month beforeharvest through harvest. The predominant disease should be listed in thetrial information and individual hybrid ratings should be given.1=No lesions to two lesions per leaf.3=A few scattered lesions on the leaf. About five to ten percent of theleaf surface is affected.5=A moderate number of lesions are on the leaf. About 15 to 20 percentof the leaf surface is affected.7=Abundant lesions are on the leaf. About 30 to 40 percent of the leafsurface is affected.9=Highly abundant lesions (>50 percent) on the leaf. Lesions are highlycoalesced. Plants may be prematurely killed.The following are disease definitions (ratings based on a 1-9 scale witha one rating indicating most resistance and a nine rating indicatingmost susceptible):

Common Rust (CR) Eye Spot (ES) Gray Leaf Spot (GLS) Northern Corn LeafBlight (NCLB) Stewart's Bacterial Wilt (SBW) Southern Corn Leaf Blight(SCLB) Southern Rust (SR) Corn Virus Complex (CVC)Preharvest Trait CodesFinal Stand (FS): count of plants per plot after thinning.Heat units to Black Layer (HUBLN): Recorded the day when 50% of allplants within a plot reach black layer stage. Converted days toaccumulated heat units from planting. Notes taken on border rows offour-row plots.Harvest Population (HAVPN): Counted the number of plants in yield rows,excluding tillers, in each plot. For FET plots, count a thousandth of anacre two times and record the average.Barren Plants (BRRNP): Counted the number of plants in yield rows havingno ears and/or abnormal ears with less than 50 kernels. For FET plots,counted barren plants out of 50 from two locations in each hybrid strip,sum, and record the percentage. Data collected on entire trial.Dropped Ears (DROPP) or (% DE): Counted the numbers of ears lying on theground in yield rows. For FET plots, count dropped ears from the area of50 plants from two locations in each hybrid strip, sum, and record thepercentage.Stalk Lodging % (STKLP) or (% SL): Stalk lodging will be reported asnumber of plants broken below the ear without pushing, excluding greensnapped plants. Record trials with approximately five percent or moreaverage stalk lodging. Counted the number of broken plants in yield rowsand converted to percent. For FET plots, counted stalk lodged plants outof 50 from two locations in each hybrid strip, sum, and recorded thepercentage.Root Lodging Late % (LRTLP) or (% LateRL): Late root lodging can usuallystart to occur about two weeks after flowering and involves lodging atthe base of the plant. Plants leaning at a 30-degree angle or more fromthe vertical are considered lodged. Counted the number of root lodgedplants in yield rows and converted to percent. For FET plots, countedroot lodged plants out of 50 from two locations in each hybrid strip,sum, and record the percentage.Push Test for Stalk and Root Quality on Erect Plants % (PSTSP) or (%Push): The push test is applied to trials with approximately fivepercent or less average stalk lodging. Plants are pushed that are notroot lodged or broken prior to the push test. Standing next to theplant, the hand is placed at the top ear and pushed to arm's length.Push one of the border rows (four-row small plot) into an adjacent plotborder row. Counted the number of plants leaning at a 30-degree angle ormore from the vertical, including plants with broken stalks prior topushing, did not count plants that have strong rinds that snap ratherthan bend over easily. For FET plots, push 50 plants from two interiorlocations of each hybrid strip, sum, and record the percentage. The goalof the push test is to identify stalk rot and stalk lodging potential,NOT ECB injury. If ECB injury was present, only did a push test on theECB trials.Data may be collected in the following manner:PUSXN: Push ten plants and enter the number of plants that do not remainupright.PSTSP: This is a percent. If you push 10 plants you can simply enter 10times the number of plants that do not remain upright (i.e. 2=20) to getthe percentage.Intactness (INTLR) or (Plantintact):1=Healthy appearance, tops unbroken5=25% of tops broken9=majority of tops brokenPlant Appearance (PLTAR): This is a visual rating based on general plantappearance taking into account all factors of intactness, pest, anddisease pressure.1=Complete plant with healthy appearance5=plants look OK9=Plants not acceptableGreen Snap (GRSNP) or (% Greensnap) or (% GS): Counted the number ofplants in yield rows that snapped below the ear due to brittlenessassociated with high winds. For FET plots, count snapped plants out of50 from two locations in each hybrid strip, sum, and record thepercentage.Stay-green (STGRP) or (% Staygreen) or (% SG): This is an assessment ofthe ability of a grain hybrid to retain green color as maturityapproaches (taken near the time of black-layer) and should not be areflection of hybrid maturity or leaf disease. Recorded % of greentissue.This may be listed as a Stay Green Rating or as a percentage.Stay Green Rating (STGRR): This is an assessment of the ability of agrain hybrid to retain green color as maturity approached (taken nearthe time of black layer or if major differences are noted later). Thisrating should not be a reflection of the hybrid maturity or leafdisease.1=solid Green Plant9=no green tissueEar/Kernel Rots (KRDSR): If ear or kernel rots are present, husk tenconsecutive ears in each plot and count the number that have evidence ofear or kernel rots, multiply by 10, and round up to the nearest ratingas described below. Identify and recorded the disease primarilyresponsible for the rot.1=No rots, 0% of the ears infected.3=Up to 10% of the ears infected.5=11 to 20% of the ears infected.7=21 to 35% of the ears infected.9=36% or more of the ears infected.Grain Quality (GRQUR): Husked back several ears after black layer stageand observed kernel cap integrity and relative amount of soft starchendosperm along the sides of kernels.1=smooth kernel caps and or 10% or less soft starch3=slight kernel wrinkles and or 30% soft starch7=moderate kernel wrinkles and or 70% soft starch9=severe kernel wrinkled and or 90% or more soft starchPreharvest Hybrid CharacteristicsEar Shape Slender, Semi-Blocky, BlockyDESHR:1=Blocky5=Semi-blocky9=SlenderEar Type Fixed, Semi-Fixed, Flex (Home location: Thin outside row, everyother plant for half of row.)EARFR:1=Flex5=Semi-flex9=FixedHusk Cover Short, Medium, LongHSKCR:1=Long5=Medium9=ShortKernel Depth Shallow, Medium, DeepKRLNR:1=Deep5=Medium9=Short (shallow)Shank Length Short, Medium, LongSHLNR:1=Short5=Medium9=LongCob Color (COBCR):1=White5=Pink9=Dark RedKernel Row Number: Enter average of 3 ears (KRRWN): The average numberof kernel rows on 3 ears.Cob diameter (COBDR): Cob diameter to be taken with template.

-   -   1: small    -   5: Medium    -   9: Large        Corn: Harvest Trait Codes        Endosperm Type: categorized as one of the following: normal        maize starch, high amylase, sweet, waxy starch, high protein,        high lysine, high oil, hard endosperm.        Number of Rows Harvested (NRHAN)        Plot Width (RWIDN)        Plot Length (RLENN)        Yield Lb/Plot (YGSMN)        Test Weight in Lb/Bu (TSTWN) or (TWT)        Moisture % (MST_P) or (Moist)        Adjusted Yield in Bu/A (YBUAN)—entered or calculated        EARAR—is the Ear Size Uniformity/Ear Appearance rating        CRDSR—stands for Crown Rot        Insect Ratings        ECB1R—is European Corn Borer (ECB) rating for leaf damage. The        rating is on a scale of 1-9 with 1 meaning that there is no        damage.        ECB2R—is European Corn Borer (ECB) second generation (2^(nd)        Gen.) rating for damage. This rating is a visual rating on a        scale of 1-9 with 1 meaning that there is no damage.        Color Traits        Anther color is yellow; if any other color is shown it is        recorded as Other. Kernel crown color is white, yellow, orange;        if any other color is shown then the color is indicated as        Other. Glume ring color is listed as red/purple; if any other        color is shown or if the ring color is inconsistent then        Other/Absent is recorded. Brace Root Color is listed as green,        reddish, purplish; if any other color is shown or if the color        is inconsistent then Other is recorded.

Color Choices: 1. light green 2. medium green 3. dark green 4. very darkgreen 5. green-yellow 6. pale yellow 7. yellow 8. yelow-orange 9. salmon10. pink-orange 11. pink 12. light red 13. cherry red 14. red 15. redand white 16. pale purple 17. purple 18. colorless 19. white 20. whitecapped 21. buff 22. tan 23. brown 24. bronze 25. variegated (describe)26. other (describe) Input Form # ABR. Description Value A1 EMRGN Finalnumber of plants per plot # A2 REGNN Region Developed: 1. Northwest 2.Northcentral # 3. Northeast 4. Southeast 5. Southcentral 6. Southwest 7.Other A3 CRTYN Cross type: 1. sc 2. dc 3. 3w 4. msc 5. m3w 6. inbred 7.rel. # line 8. other A4 KRTPN Kernel type: 1. sweet 2. dent 3. flint 4.flour 5. pop # 6. ornamental 7. pipecorn 8. other A5 EMERN Days toEmergence EMERN #Days B1 ERTLP % Root lodging: (before anthesis): #% B2GRSNP % Brittle snapping: (before anthesis): #% C1 TBANN Tassel branchangle of 2nd primary lateral branch (at degree anthesis): C10 HUPSN Heatunits to 50% pollen shed: (from emergence) #HU C11 SLKCN Silk color:#/Munsell value C12 HU5SN Heat units to 50% silk: (from emergence) #HUC13 DSAZN Days to 50% silk in adapted zone: #Days C14 HU9PN Heat unitsto 90% pollen shed: (from emergence) #HU C15 HU19N Heat units from 10%to 90% pollen shed: #HU C16 DA19N Days from 10% to 90% pollen shed:#Days C2 LSPUR Leaf sheath pubescence of second leaf above the ear (at #anthesis) 1-9 (1 = none): C3 ANGBN Angle between stalk and 2nd leafabove the ear (at degree anthesis): C4 CR2LN Color of 2nd leaf above theear (at anthesis): #/Munsell value C5 GLCRN Glume Color: #/Munsell valueC6 GLCBN Glume color bars perpendicular to their veins (glume #bands): 1. absent 2. present C7 ANTCN Anther color: #/Munsell value C8PLQUR Pollen Shed: 1-9 (0 = male sterile) # C9 HU1PN Heat units to 10%pollen shed: (from emergence) #HU D1 LAERN Number of leaves above thetop ear node: # D10 LTBRN Number of lateral tassel branches thatoriginate from the # central spike: D11 EARPN Number of ears per stalk:# D12 APBRR Anthocyanin pigment of brace roots: 1. absent 2. faint # 3.moderate 4. dark D13 TILLN Number of tillers: # D14 HSKCN Husk color 25days after 50% silk: (fresh) #/Munsell value D2 MLWVR Leaf marginalwaves: 1-9 (1 = none) # D3 LFLCR Leaf longitudinal creases: 1-9 (1 =none) # D4 ERLLN Length of ear leaf at the top ear node: #cm D5 ERLWNWidth of ear leaf at the top ear node at the widest point: #cm D6 PLHTNPlant height to tassel tip: #cm D7 ERHCN Plant height to the top earnode: #cm D8 LTEIN Length of the internode between the ear node and the#cm node above: D9 LTASN Length of the tassel from top leaf collar totassel tip: #cm E1 HSKDN Husk color 65 days after 50% silk: (dry)#/Munsell value E10 DSGMN Days from 50% silk to 25% grain moisture inadapted #Days zone: E11 SHLNN Shank length: #cm E12 ERLNN Ear length:#cm E13 ERDIN Diameter of the ear at the midpoint: #mm E14 EWGTN Weightof a husked ear: #gm E15 KRRWR Kernel rows: 1. indistinct 2. distinct #E16 KRNAR Kernel row alignment: 1. straight 2. slightly curved # 3.curved E17 ETAPR Ear taper: 1. slight 2. average 3. extreme # E18 KRRWNNumber of kernel rows: # E19 COBCN Cob color: #/Munsell value E2 HSKTRHusk tightness 65 days after 50% silk: 1-9 (1 = loose) # E20 COBDNDiameter of the cob at the midpoint: #mm E21 YBUAN Yield: #kg/ha E22KRTEN Endosperm type: 1. sweet 2. extra sweet 3. normal 4. high 3amylose 5. waxy 6. high protein 7. high lysine 8. super sweet 9. highoil 10. other E23 KRCLN Hard endosperm color: #/Munsell value E24 ALECNAleurone color: #/Munsell value E25 ALCPR Aleurone color pattern: 1.homozygous 2. segregating # E26 KRLNN Kernel length: #mm E27 KRWDNKernel width: #mm E28 KRDPN Kernel thickness: #mm E29 K1KHN 100 kernelweight: #gm E3 HSKCR Husk extension: 1. short (ear exposed) 2. medium (8cm) # 3. long (8-10 cm) 4. very long (>10 cm) E30 KRPRN % round kernelson 13/64 slotted screen: #% E4 HEPSR Position of ear 65 days after 50%silk: 1. upright # 2. horizontal 3. pendent E5 STGRP Staygreen 65 daysafter anthesis: 1-9 (1 = worst) # E6 DPOPP % dropped ears 65 days afteranthesis: % E7 LRTRP % root lodging 65 days after anthesis: % E8 HU25NHeat units to 25% grain moisture: (from emergence) #HU E9 HUSGN Heatunits from 50% silk to 25% grain moisture in #HU adapted zone:

DETAILED DESCRIPTION OF THE INVENTION

G07-NPID5459 is shown in comparison with NP2357.

The inbred provides uniformity and stability within the limits ofenvironmental influence for traits as described in the VarietyDescription Information (Table 1) that follows.

The inbred has been produced through a dihaploid system or isself-pollinated for a sufficient number of generations to give inbreduniformity. During plant selection in each generation, the uniformity ofplant type was selected to ensure homozygosity and phenotypic stability.The line has been increased in isolated farmland environments with dataon uniformity and agronomic traits being observed to assure uniformityand stability. No variant traits have been observed or are expected inG07-NPID5459.

The best method of producing the invention is by planting the seed ofG07-NPID5459 which is substantially homozygous and self-pollinating orsib pollinating the resultant plant in an isolated environment, andharvesting the resultant seed.

TABLE 1 G07-NPID5459 VARIETY DESCRIPTION INFORMATION #1 Type: Dent #2Region Best Adapted: Broadly adapted MG Maturity Hybrid RM Group Range(estimate) 5 103-107 106 #3 Plant Traits AntherClr GlumeClr SilkClrBraceRtClr CobClr KernelClr Yellow Green Pink Absent to Faint Red Yellow

The data provided above is often a color. The Munsell code is areference book of color, which is known and used in the industry and bypersons with ordinary skill in the art of plant breeding. The purity andhomozygosity of inbred G07-NPID5459 is constantly being tracked usingisozyme genotypes.

Table 2 shows a comparison between G07-NPID5459 and a comparable inbred.

G07-NPID5459 and NP2357 have similar pollen quantities and shedduration.

TABLE 2 PAIRED INBRED COMPARISON DATA Pollen % Poll. Shed % Shed YearInbred Quant. Quant. Duration Dur. Overall G07-NPID5459 1589926 84 175.396.3 NP2357 884740.7 46.3 156.3 86 Diff 705185.3 37.7 19 10.3 # Expts 33 4 4 Prob 0.503 0.506 0.514 0.529 *.05 < Prob <= .10 **.01 < Prob <=.05 ***.00 < Prob <= .01

Table 3 shows the GCA (General Combining Ability) estimates ofG07-NPID5459 compared with the GCA estimates of the other inbreds. Theestimates show the general combining ability is weighted by the numberof experiment/location combinations in which the specific hybridcombination occurs. The interpretation of the data for all traits isthat a positive comparison is a practical advantage. A negativecomparison is a practical disadvantage. The general combining ability ofan inbred is clearly evidenced by the results of the general combiningability estimates. This data compares the inbred parent in a number ofhybrid combinations to a group of “checks”. The check data is from ourcompany's and other companies' hybrids which are commercial products andpre-commercial hybrids, which were grown in the same sets and locations.

TABLE 3 Parent1 Parent2 N05 N06 N07 N Yield Moist TWT PCTERL PCTSLPCTPUSH PLTLRL PCTDE FS PCTSG G07-NPID5459 25 25 7.58 −0.45 −0.22 0.3112.96 −2.3 −0.66 0.31 G07-NPID5459 11 11 8.67 −2.23 −0.04 −4.29 1.98−0.3 G07-NPID5459 10 10 1.09 0.01 0.23 −0.18 1.98 0 G07-NPID5459 11 118.17 −2.15 −0.22 2.33 3.5 0.6 G07-NPID5459 26 26 −11.76 0.38 0.02 2.091.3 −6.15 0.18 0.23 G07-NPID5459 24 37 43 104 7.03 −0.05 0.13 3.96 −0.259.6 2.19 0.02 0.73 −5.99 G07-NPID5459 10 10 −9.7 −0.73 −0.12 −1.64 3.50.15 G07-NPID5459 15 15 −13.51 −0.45 −0.1 −15.07 1.27 −0.33 G07-NPID545915 15 −9.08 −1.32 −0.15 −1.14 3.98 −0.33 G07-NPID5459 15 15 1.28 −1.14−0.19 0.17 1.24 −0.33 G07-NPID5459 14 14 8.97 −4.07 −0.32 −0.58 0.93−0.36 G07-NPID5459 10 10 13.09 −2.35 −0.01 −3.03 3.5 −0.4 G07-NPID545910 10 7.13 −1.02 −0.02 −0.03 −1.05 −0.03 G07-NPID5459 11 11 13.17 −0.450.04 1.5 3.5 0.42 G07-NPID5459 11 11 2.26 −2.62 −0.04 −6.48 3.5 0.15G07-NPID5459 10 10 1.38 −2.03 −0.12 2.33 3.5 0.17 G07-NPID5459 10 107.13 −2.37 −0.16 2.33 −7.11 −0.21 XR = 10 363 3.07 −0.86 −0.02 3.96−0.99 8.5 0.47 −0.1 0.24 −5.99 XH = 10 17 2.52 −1.36 −0.08 3.96 −1.277.95 1.06 −0.15 0.03 −5.99 XT = 10 1 7.03 −0.05 0.13 3.96 −0.25 9.6 2.190.02 0.73 −5.99 Parent1 Parent2 PCTGS Pop Emerge Vigor PlantIntact HUS50HUBL Pltht Earht G07-NPID5459 148.67 0.43 0.2 0.81 50.4 4.36 2.23G07-NPID5459 −147.23 0.44 3.44 4.44 27.22 G07-NPID5459 0 −1.56 −4.5619.44 −7.78 G07-NPID5459 292.78 0.44 1.44 14.44 12.22 G07-NPID5459111.44 −0.29 0.7 0.43 −5.44 −9.84 −0.37 G07-NPID5459 0.25 371.45 1.06−0.54 1 11.85 −2542.9 3.38 12.26 G07-NPID5459 72.77 −1.56 1.44 4.44 7.22G07-NPID5459 −161.34 0.05 50.28 −18.67 −9.33 G07-NPID5459 −161.34 −0.4550.28 −10.33 −11 G07-NPID5459 −161.34 −0.7 67.68 −20.23 −16 G07-NPID5459−172.86 −0.45 113.68 6.33 4 G07-NPID5459 −191.23 −1.56 −0.56 4.44 7.22G07-NPID5459 −16.75 0.44 1.44 −0.56 −2.78 G07-NPID5459 204.77 0.44 −2.56−0.56 12.22 G07-NPID5459 72.77 0.44 −2.56 −0.56 −2.78 G07-NPID5459 80.05−1.56 1.44 14.44 2.22 G07-NPID5459 −103.23 −1.56 −0.56 −5.56 −2.78 XR =0.25 118.18 0.42 −0.29 0.71 24.33 −2542.9 −0.82 3.7 XH = 0.25 14.08−0.05 −0.4 0.05 48.39 −2542.9 0.55 2 XT = 0.25 371.45 1.06 −0.54 1 11.85−2542.9 3.38 12.26 XR = GCA Estimate: Weighted by Expt XH = GCAEstimate: Weighted by Parent2 XT = Same as XH but using only thoseparent2 with two years of data

Table 4 shows the inbred G07-NPID5459 in hybrid combination, incomparison with another hybrid, which is adapted for the same region ofthe Corn Belt. When in this hybrid combination, the G07-NPID5459 hybridcarries significantly more yield and moisture in comparison to the otherhybrid. The test weight for the hybrid and the present invention hybridis significantly different. The two hybrids show similar agronomictraits when grown together in this environment except for thesignificantly different final stand (FS).

TABLE 4 PAIRED HYBRID COMPARISON DATA Year Hybrid Yield Moist TWT % SL %Push % LateRL % EarlyRL % DE FS % StayGreen Overall G07-NPID5459 204.919.8 55.8 3.3 10.0 0.7 0.0 0.0 62.9 30.9 Hybrid Hybrid 2 190.5 18.9 56.44.4 19.2 1.4 0.0 0.0 64.6 25.0 #Expts 61.0 61.0 54.0 27.0 6.0 14.0 5.04.0 61.0 11.0 Diff 14.4 0.9 0.6 1.1 9.2 0.8 0.0 0.0 1.7 5.9 Prob0.000*** 0.000*** 0.004*** 0.2 0.5 0.5 0.000*** 0.2 Heatunits HeatunitsYear Hybrid % Greensnap % Barren Emerge Vigor to S50 to P50 Earht PlthtOverall G07- 1.3 1.7 5.4 4.1 1309.0 1292.0 119.6 278.4 NPID5459 HybridHybrid 2 1.0 0.0 5.3 4.4 1316.0 1294.0 101.8 273.8 #Expts 4.0 3.0 17.014.0 8.0 8.0 10.0 10.0 Diff 0.4 1.7 0.1 0.3 6.8 1.2 17.8 4.7 Prob 0.70.4 0.5 0.2 0.5 0.9 0.000*** 0.4 *.05 < Prob <= .10 **.01 < Prob <= .05***.00 < Prob <= .01

Table 5 shows the yield response of G07-NPID5459 in hybrid combinationin comparison with the plants in the environment around it at the samelocation and in comparison to hybrid 2. The data for the present hybridis subject to an error of 21.4 and hybrid 2 has an error of 22.2. Evenwith this large of an error the hybrid of the present invention isoverachieving yields in the low to mid environments.

TABLE 5 YIELD RESPONSE Research Plots # Hybrid Error Plots 75 100 125150 175 200 G07-NPID5459 21.4 61 108 128 148 169 189 209 Hybrid Hybrid 222.2 307 63 90 118 145 173 200

This invention also is directed to methods for producing a corn plant bycrossing a first parent corn plant with a second parent corn plantwherein the first or second parent corn plant is an inbred corn plantfrom the line G07-NPID5459. Further, both first and second parent cornplants can come from the inbred corn line G07-NPID5459 which produces aself of the inbred invention. The present invention can be employed in avariety of breeding methods which can be selected depending on the modeof reproduction, the trait, and the condition of the germplasm. Thus,any breeding methods using the inbred corn line G07-NPID5459 are part ofthis invention: selfing, backcrosses, hybrid production, and crosses topopulations, and haploid by such old and known methods of using KWSinducers lines, Krasnador inducers, stock six material that induceshaploids and anther culturing and the like.

The present invention may be useful as a male-sterile plant. Sterilitycan be produced by pulling or cutting tassels from the plant,detasseling, use of gametocides, use of genetic material to render theplant sterile using a CMS type of genetic control or a nuclear geneticsterility. Male sterility is employed in a hybrid production byeliminating the pollen from the seed producing parent so when inisolation from other pollen source the only available pollen is thatfrom the second male fertile inbred planted most often in rows near themale sterile inbred.

Methods for genetic male sterility are disclosed in EPO 89/3010153.8, WO90/08828, U.S. Pat. Nos. 4,654,465, 4,727,219, 3,861,709, 5,432,068 and3,710,511. Gametocides which are chemicals or substances that negativelyaffect the pollen or at least the fertility of the pollen can beemployed to provide male sterility.

Unfortunately, for hybrid production nature complicates male sterilityand as a result there are self pollinated female inbred seeds in somehybrid production. Great measures are taken to avoid this inbredproduction in a hybrid field but it unfortunately does occur. If ahybrid bag of seed is tested with molecular markers it may be possibleto detect inbred seed. If the hybrid seed is planted these inbred plantstend to be readily identifiable as runt like appearance—shorter plant,small ear, or other characteristics when the hybrid seed in a bag isplanted. Self pollination of these plants produces the female inbredseed. The seed in a hybrid seed bag is not sold to the growers forbreeding but to plant and produce only for use as grain and forage.

Process for producing seed comprises planting a group of seed comprisingseed from a hybrid production, one of whose parents is the presentinvention said group, growing plants from this seed, identifying anyinbred plants, selecting and pollinating the inbred plant.

A number of well known methods can be employed to identify the genotypeof maize. The ability to understand the genotype of the presentinvention increases as the technology moves toward better markers foridentifying different components within the maize genetic material. Oneof the oldest methods is the use of isozymes which provides ageneralized footprint of the material. Other markers that were adaptedto provide a higher definition profile include Restriction FragmentLength Polymorphisms (RFLPs), Amplified Fragment Length Polymorphisms(AFLPs), Random Amplified Polymorphic DNAs (RAPDs), Polymerase ChainReaction (there are different types of primers or probes) (PCR),Microsattelites (SSRs), and Single Nucleotide Polymorphisms (SNPs) justto list a few. The use of these and a number of other markers forgathering genotype information is well understood in the industry andcan be found in college textbooks such as Breeding Field Crops, Miltonet. al., Iowa State University Press.

The profile of the inbred of this invention should be close tohomozygous for alleles. A marker profile produced with any of the locusidentifying systems known in the industry will identify a particularallele at particular loci. A F1 hybrid made from the inbred of thisinvention will comprise a marker profile of the sum of both of itsinbred parents. At each locus the allele for the present invention andthe allele for the other inbred parent should be present. Thus theprofile of the present invention will permit identification of hybridsas containing the inbred parent of the present invention. To identifythe female portion of the hybrid the material from the pericarp which ismaternally inherited is employed. The comparison of this maternalprofile with the hybrid profile will allow identification of thepaternal profile. The present invention includes a maize cell that ispart of an inbred or hybrid plant which includes its seed or plant partthat has the marker profile of alleles of the present invention.

Marker systems are not just useful for identification of the presentinvention; they are also useful for breeding and trait conversiontechniques. Polymorphisms in maize permit the use of markers for linkageanalysis. If SSR are employed with flanking primers PCR can be used andSouthern Blots can often be eliminated. Use of flanking markers and PCRand amplification of the material is well known by the industry. Primersfor SSRS and mapping information are publicly available through the helpof the USDA at Maize GDB on the web.

Marker profiles of this invention can identify essentially derivedvarieties or progeny developed with the inbred in its ancestry. Thisinbred may have progeny identified by having a molecular marker profileof at least 25%, to 40%, 45%, 50% to 80% (which includes each of thenumbers between these two percentages), 81%, 82%, 83%, 84%, 85%, 86%,87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% geneticcontribution of the present inbred invention, as measured by eitherpercent identity or percent similarity.

The present invention may have a new locus or trait introgressed throughdirect transformation or backcrossing or marker assisted breeding. Abackcross conversion or locus conversion both refer to a product of abackcrossing program.

DNA sequences are introduced through backcrossing (Hallauer et al. inCorn and Corn Improvement, Sprague and Dudley, Third Ed. 1998), withPH8JV utilized as the recurrent parent.

When the present inbred is used as a recurrent parent in a breedingprogram it is often referred to as backcrossing. Backcrossing is oftenemployed to introgression a desired trait or trait(s), either transgenicor nontransgenic, into the recurrent parent. A plant may be selectedwith the trait or the desired locus in one or more backcrosses. Ifmarkers are employed to assist in selection the number of backcrossesneeded to recover the recurrent parent with the desired trait or locuscan be relatively few two or three. However, three, four, five or morebackcrosses are often required to produce the desired inbred with thegene or loci conversion in place. The number of backcrosses needed for atrait introgression is often linked to the genetics of the trait.Multigenic traits, recessive alleles, unlinked traits, how the traitsare inherited all will play a role in the number of backcrosses that maybe necessary to achieve the desired backcross conversion of the inbred.

Dominant, single gene traits or traits with obvious phenotypic changesare particularly well managed in a backcrossing program. Prior totransformation and prior to markers, backcrossing was employed since atleast the 1950's to alter grain color, to move mutations intoinbreds—such as sugary 2, waxy, amylose extender, dull, brittle,shrunken, sugary 1, waxy (wx), shrunken-2.

In a book written by Dr. Hallauer, entitled Corn and Corn Improvement,published by Sprague and Dudley, 3rd Ed. (1998), the basics ofbackcrossing along with a number of other corn breeding methods such asrecurrent or bulk or mass selection, pedigree breeding, open pollinationbreeding, marker assisted selection, double haploids development andbreeding is taught. The ordinary corn breeder understands these breedingsystems and how to apply them to the present invention; therefore,repetition of these breeding methods need not be listed within thisapplication.

The backcrossing program is more complicated when the trait is arecessive gene. To determine the presence of the recessive gene oftenrequires the use of additional testing to determine if the trait hasbeen transferred. Use of markers to detect the gene reduces thecomplexity of trait identification in the progeny. A marker that is aSNP specific for the trait itself can be very useful in increasing theefficiency and speed of tracking a recessive trait within a backcrossingprogram. Mutations for the last 60 years have been backcrossed in thismanner into elite germplasm.

Mutations can be induced in germplasm by the plant breeder. Mutationscan result from plant or seed or pollen exposure to temperaturealterations, culturing, radiation in various forms, chemical mutagenslike EMS and others. Some of the mutant genes which have been identifiedin maize include the genotypes: waxy (wx), amylose extender (ae), dull(du), horny (h), shrunken (sh), brittle (bt), floury (fl), opaque (O),and sugary (su). Nomenclature for mutant genes is based on the effectthese mutant genes have on the physical appearance, phenotype, of thekernel. It is also known that within these genotypes there are geneswhich produce starch with markedly different functional properties eventhough the phenotypes are the same. Such subspecies have generally beengiven a number after the named genotype, for example, sugary-1 (su1),sugary-2 (su2); shrunken 1 and shrunken 2. Traits such as Ht, waxy,shrunken, amylose extender, opaque, sugary 1, 2, dull, IT, IR,sterility, fertility, low phytic acid, NLB, SLB, and the like have allbeen introgressed into elite inbreds through backcrossing programs. Thelast backcross generation may be selfed if necessary to recover theinbred of interest with the introgressed trait.

All plants and plant cells produced using inbred corn line G07-NPID5459are within the scope of this invention. The invention encompasses theinbred corn line used in crosses with other, different, corn inbreds toproduce (F1) corn hybrid seeds and hybrid plants and the grain producedon the hybrid plant. This invention includes plant and plant cells,which upon growth and differentiation produce corn plants having thephysiological and morphological characteristics of the inbred lineG07-NPID5459.

Additionally, this maize line can, within the scope of the invention,contain: a mutant gene such as, but not limited to, amylose, amylase,sugary 1, shrunken 1, waxy, AE (amylose extender), dull, brown midrib,or imazethapyr tolerant (IT or IR™); or transgenes such as, but notlimited to, insect resistant genes such as Corn Rootworm gene(s) in theevent DAS-59122-7, Mir603 Modified Cry3A event, MON 89034, MON 88017Bacillus thuringiensis (Cry genes) Cry34/35Ab1, Cry1A.105, PO Cry1F,Cry2Ab2, Cry1A, Cry1AB, Cry1Ac Cry3Bb1, or herbicide resistant genessuch as pat gene or bar gene, EPSP, the altered protoporphyrinogenoxidase (protox enzyme) U.S. Pat. Nos. 5,767,373; 6,282,837; WO01/12825, or disease resistant genes such as the Mosaic virus resistantgene, etc., or other trait altering genes such as lignin genes,flowering genes, oil modifying genes, senescence genes and the like.Altered carbohydrates or altered starch can include genes for enzymesthat affects the synthases, branching enzymes, pullanases, debranchingenzymes, isoamylases, alpha amylases, beta amylases, AGP, ADP and otherenzymes which effect the amylose, amylopectin ratio or content or thebranching pattern of starch. The fatty acid modifying genes can alsoaffect starch content.

The methods and techniques for inserting, or producing and/oridentifying a mutation or making or reshuffling a transgene andintrogressing the trait or gene into the present invention throughbreeding, transformation, mutating and the like are well known andunderstood by those of ordinary skill in the art.

A number of different inventions exist which are designed to avoiddetasseling in maize hybrid production. Some examples are switchablemale sterility, lethal genes in the pollen or anther, inducible malesterility, male sterility genes with chemical restorers, sterility geneslinked with a parent. U.S. Pat. No. 6,025,546 relates to the use oftapetum-specific promoters and the barnase gene. U.S. Pat. No. 6,627,799relates to modifying stamen cells to provide male sterility. Therefore,one aspect of the current invention concerns the present inventioncomprising one or more gene(s) capable of restoring male fertility tomale-sterile maize inbreds or hybrids.

Various techniques for breeding, moving or altering genetic materialwithin or into the present invention (whether it is an inbred or inhybrid combination) are also known to those skilled in the art. Thesetechniques (to list only a few) are anther culture for haploid/doublehaploid production, stock six, (which is a breeding/selection methodusing color markers and is a method that has been in use for forty yearsand is well known to those with skill in the art), transformation,irradiation to produce mutations, chemical or biological mutation agentsand a host of other methods are within the scope of the invention. Allparts of the G07-NPID5459 plant including plant cells are within thescope of this invention. The term transgenic plant refers to plantshaving genetic sequences, which are introduced into the genome of aplant by a transformation method and the progeny thereof. Transformationmethods are means for integrating new genetic coding sequences into theplant's genome by the incorporation of these sequences into a plantthrough man's assistance, but not by breeding practices. The transgeneonce introduced into plant material and integrated stably can be movedinto other germplasm by standard breeding practices.

Though there are a large number of known methods to transform plants,certain types of plants are more amenable to transformation than areothers. Monocots can present some transformation challenges, however,the basic steps of transforming monocots plants have been known in theart for about 15 years. The most common method of maize transformationis referred to as gunning or microprojectile bombardment though othermethods can be used. The process employs small gold-coated particlescoated with DNA which are shot into the transformable material. Detailedtechniques for gunning DNA into cells, tissue, callus, embryos, and thelike are well known in the prior art. One example of steps that can beinvolved in monocot transformation are concisely outlined in U.S. Pat.No. 5,484,956 “Fertile Transgenic Zea mays Plants ComprisingHeterologous DNA Encoding Bacillus Thuringiensis Endotoxin” issued Jan.16, 1996 and also in U.S. Pat. No. 5,489,520 “Process of ProducingFertile Zea mays Plants and Progeny Comprising a Gene EncodingPhosphinothricin Acetyl Transferase” issued Feb. 6, 1996.

Plant cells such as maize can be transformed not only by the use of agunning device but also by a number of different techniques. Therecombinant DNA molecules of the invention can be introduced into theplant cell in a number of art-recognized ways. Those skilled in the artwill appreciate that the choice of method might depend on the type ofplant, i.e. monocot or dicot, targeted for transformation. Suitablemethods of transforming plant cells include microinjection (Crossway etal., BioTechniques 4:320-334 (1986)), electroporation (Riggs et al,Proc. Natl. Acad. Sci. USA 83:5602-5606 (1986), Agrobacterium mediatedtransformation (Hinchee et al., Biotechnology 6:915-921 (1988)), directgene transfer (Paszkowski et al., EMBO J. 3:2717-2722 (1984)), ballisticparticle acceleration using devices available from Agracetus, Inc.,Madison, Wis. and Dupont, Inc., Wilmington, Del. (see, for example,Sanford et al., U.S. Pat. No. 4,945,050; and McCabe et al.,Biotechnology 6:923-926 (1988)), protoplast transformation/regenerationmethods (see U.S. Pat. No. 5,350,689 issued Sep. 27, 1994 to Ciba-GeigyCorp.), Whiskers technology (See U.S. Pat. Nos. 5,464,765 and 5,302,523)and pollen transformation (see U.S. Pat. No. 5,629,183). Also see,Weissinger et al., Annual Rev. Genet. 22:421-477 (1988); Sanford et al.,Particulate Science and Technology 5:27-37 (1987)(onion); Christou etal., Plant Physiol. 87:671-674 (1988)(soybean); McCabe et al.,Bio/Technology 6:923-926 (1988)(soybean); Datta et al., Bio/Technology8:736-740 (1990)(rice); Klein et al., Proc. Natl. Acad. Sci. USA,85:4305-4309 (1988)(maize); Klein et al., Bio/Technology 6:559-563(1988)(maize); Klein et al., Plant Physiol. 91:440-444 (1988)(maize);Fromm et al., Bio/Technology 8:833-839 (1990); Gordon-Kamm et al., PlantCell 2:603-618 (1990)(maize); and U.S. Pat. Nos. 5,591,616 and 5,679,558(rice).

A further subject of the present invention are the plants which comprisetransformed cells, in particular the plants regenerated from transformedcells. Regeneration is effected by any suitable process, which dependson the nature of the species as described, for example, in thereferences hereinabove. Patents and patent applications which are citedin particular for the processes for transforming plant cells andregenerating plants are the following: U.S. Pat. Nos. 4,459,355,4,536,475, 5,464,763, 5,177,010, 5,187,073, EP 267,159, EP 604 662, EP672 752, U.S. Pat. Nos. 4,945,050, 5,036,006, 5,100,792, 5,371,014,5,478,744, 5,179,022, 5,565,346, 5,484,956, 5,508,468, 5,538,877,5,554,798, 5,489,520, 5,510,318, 5,204,253, 5,405,765, EP 442 174, EP486 233, EP 486 234, EP 539 563, EP 674 725, WO 91/02071 and WO95/06128.

The use of pollen, cotyledons, zygotic embryos, meristems and ovum asthe target issue can eliminate the need for extensive tissue culturework. Generally, cells derived from meristematic tissue are useful. Themethod of transformation of meristematic cells of cereal is taught inthe PCT application WO96/04392. Any number of various cell lines,tissues, calli and plant parts can and have been transformed by thosehaving knowledge in the art. Methods of preparing callus or protoplastsfrom various plants are well known in the art and specific methods aredetailed in patents and references used by those skilled in the art.Cultures can be initiated from most of the above-identified tissue. Theonly true requirement of the transforming plant material is that it canultimately be used to form a transformed plant.

Heterologous means of different natural origin or represents anon-natural state. A host cell transformed with a nucleotide sequencederived from another organism, particularly from another species, thatnucleotide sequence is heterologous with respect to that host cell anddescendants. Heterologous refers to a nucleotide sequence derived fromand inserted into the same natural, original cell type, but which ispresent in a non-natural state, e.g. a different copy number, or underthe control of different regulatory sequences. A transforming nucleotidesequence may comprise a heterologous coding sequence, or heterologousregulatory sequences. Alternatively, the transforming nucleotidesequence may be completely heterologous or may comprise any possiblecombination of heterologous and endogenous nucleic acid sequences.

The DNA used for transformation of these plants clearly may be circular,linear, and double or single stranded. The DNA is placed within aplasmid. The plasmid usually contains regulatory and/or targetingsequences which assists the expression or targeting of the gene in theplant. The methods of forming plasmids for transformation are known inthe art. Plasmid components can include such items as: leader sequences,transit polypeptides, promoters, terminators, genes, introns, markergenes, etc. The gene orientations can be sense, antisense, partialantisense or partial sense; and, multiple gene copies can be used. Thetransgene can come from plants or be from various non-plant genes suchas; bacteria, yeast, animals, and viruses.

The regulatory promoters employed can be constitutive such as CaMv35S(usually for dicots) and polyubiquitin for monocots or tissue specificpromoters such as CAB promoters, MR7 described in U.S. Pat. No.5,837,848, etc. The prior art promoters, includes but is not limited to,octopine synthase, nopaline synthase, CaMv19S, mannopine synthase. Theseregulatory sequences can be combined with introns, terminators,enhancers, leader sequences and the like in the material used fortransformation.

The isolated DNA is then transformed into the plant. A transgeneintrogressed into this invention typically comprises a nucleotidesequence whose expression is responsible or contributes to the traitunder the control of a promoter appropriate for the expression of thenucleotide sequence at the desired time in the desired tissue or part ofthe plant. Constitutive or inducible promoters are used. The transgenemay also comprise other regulatory elements, such as, for exampletranslation enhancers or termination signals. In an embodiment, thenucleotide sequence is the coding sequence of a gene and is transcribedand translated into a protein. In another embodiment, the nucleotidesequence encodes an antisense RNA, a sense RNA that is not translated oronly partially translated, a t-RNA, a r-RNA or a sn-RNA.

The genes responsible for a specific gene trait are generally inheritedthrough the nucleus. Known exceptions are, e.g. the genes for malesterility, some of which are inherited cytoplasmically, but still act assingle gene traits. In one embodiment, a heterologous transgene to betransferred to present invention is integrated into the nuclear genomeof the donor, non-recurrent parent. In another embodiment, aheterologous transgene to be transferred into the present invention isintegrated into the plastid genome of the donor, non-recurrent parent.

In an embodiment, a transgene whose expression results or contributes toa desired trait to be transferred to the present invention comprises avirus resistance trait such as, for example, a MDMV strain B coatprotein gene whose expression confers resistance to mixed infections ofmaize dwarf Mosaic virus and maize Chlorotic Mottle virus in transgenicmaize plants (Murry et al. Biotechnology (1993) 11:1559 64). In anotherembodiment, a transgene introgressed into the present inventioncomprises a gene encoding an insecticidal protein, such as, for example,a crystal protein of Bacillus thuringiensis or a vegetative insecticidalprotein from Bacillus cereus, such as VIP3 (see for example Estruch etal. Nat Biotechnol (1997) 15:137 41. Also see, U.S. Pat. Nos. 5,877,012,6,291,156; 6,107,279, 6,291,156 and 6,429,360. In another embodiment, aninsecticidal gene introduced into present invention is a Cry1Ab gene ora portion thereof, for example introgressed into present invention froma maize line comprising a Bt-11 event as described in U.S. Pat. No.6,114,608, which is incorporated herein by reference, or from a maizeline comprising a 176 event as described in Koziel et al. (1993)Biotechnology 11: 194 200. In yet another embodiment, a transgeneintrogressed into present invention comprises a herbicide tolerancegene. For example, expression of an altered acetohydroxyacid synthase(AHAS) enzyme confers upon plants tolerance to various imidazolinone orsulfonamide herbicides (U.S. Pat. No. 4,761,373). In another embodiment,a non-transgenic trait conferring tolerance to imidazolinones isintrogressed into present invention (e.g. a “IT” or “IR” trait). U.S.Pat. No. 4,975,374, incorporated herein by reference, relates to plantcells and plants containing a gene encoding a mutant glutaminesynthetase (GS) resistant to inhibition by herbicides that are known toinhibit GS, e.g. phosphinothricin and methionine sulfoximine. Also,expression of a Streptomyces bar gene encoding a phosphinothricin acetyltransferase in maize plants results in tolerance to the herbicidephosphinothricin or glufosinate (U.S. Pat. No. 5,489,520). U.S. Pat. No.5,013,659, which is incorporated herein by reference, is directed toplants that express a mutant acetolactate synthase (ALS) that rendersthe plants resistant to inhibition by sulfonylurea herbicides. U.S. Pat.No. 5,162,602 discloses plants tolerant to inhibition bycyclohexanedione and aryloxyphenoxypropanoic acid herbicides. Thetolerance is conferred by an altered-acetyl coenzyme A carboxylase(ACCase). U.S. Pat. No. 5,554,798 discloses transgenic glyphosatetolerant maize plants, which tolerance is conferred by an altered5-enolpyruvyl-3-phosphoshikimate (EPSP) synthase gene. U.S. Pat. No.5,804,425 discloses transgenic glyphosate tolerant maize plants, whichtolerance is conferred by an EPSP synthase gene derived fromAgrobacterium tumefaciens CP-4 strain. Also, tolerance to aprotoporphyrinogen oxidase inhibitor is achieved by expression of atolerant protoporphyrinogen oxidase enzyme in plants (U.S. Pat. No.5,767,373). Another trait transferable to the present invention confersa safening effect or additional tolerance to an inhibitor of the enzymehydroxyphenylpyruvate dioxygenase (HPPD) and transgenes conferring suchtrait are, for example, described in WO 9638567, WO 9802562, WO 9923886,WO 9925842, WO 9749816, WO 9804685 and WO 9904021. All issued patentsreferred to herein are, in their entirety, expressly incorporated hereinby reference.

In an embodiment, a transgene transferred to present invention comprisesa gene conferring tolerance to a herbicide and at least anothernucleotide sequence encoding another trait, such as for example, aninsecticidal protein. Such combination of single gene traits is forexample a Cry1Ab gene and a bar gene.

By way of example only, specific events (followed by their APHISpetition numbers) that can be transformed or introgressed into maizeplants include the glyphosate tolerant event GA21 (97-09901p) or theglyphosate tolerant event NK603 (00-011-01p), the glyphosatetolerant/Lepidopteran insect resistant event MON 802 (96-31701p) Mon810,Lepidopteran insect resistant event DBT418 (96-29101p), male sterileevent MS3 (95-22801p), Lepidopteran insect resistant event Bt11(95-19501p), phosphinothricin tolerant event B16 (95-14501p),Lepidopteran insect resistant event MON 80100 (95-09301p) and MON 863(01-137-01p), phosphinothricin tolerant events T14, T25 (94-35701p),Lepidopteran insect resistant event 176 (94-31901p) and Western cornrootworm (04-362-01p), and the phosphinothricin tolerant andLepidopteran insect resistant event CBH-351 (92-265-01p).

After the transformation of the plant material is complete, the nextstep is identifying the cells or material, which has been transformed.In some cases, a screenable marker is employed such as thebeta-glucuronidase gene of the uidA locus of E. coli. Then, thetransformed cells expressing the colored protein are selected. In manycases, a selectable marker identifies the transformed material. Theputatively transformed material is exposed to a toxic agent at varyingconcentrations. The cells not transformed with the selectable marker,which provides resistance to this toxic agent, die.

Cells or tissues containing the resistant selectable marker generallyproliferate. It has been noted that although selectable markers protectthe cells from some of the toxic affects of the herbicide or antibiotic,the cells may still be slightly affected by the toxic agent by havingslower growth rates. If the transformed material was cell lines thenthese lines are regenerated into plants. The cells' lines are treated toinduce tissue differentiation. Methods of regeneration of cellular maizematerial are well known in the art.

As used herein, the term “plant” includes plant cells, plantprotoplasts, plant cell tissue cultures from which maize plants can beregenerated, plant calli, plant clumps, and plant cells that are intactin plants or parts of plants, such as embryos, pollen, ovules, flowers,kernels, ears, cobs, leaves, husks, stalks, roots, root tips, anthers,silk, seeds and the like.

Duncan, Williams, Zehr, and Widholm, Planta (1985) 165:322 332 reflectsthat 97% of the plants cultured that produced callus were capable ofplant regeneration. Subsequent experiments with both inbreds and hybridsproduced 91% regenerable callus that produced plants. In a further studyin 1988, Songstad, Duncan & Widholm in Plant Cell Reports (1988), 7:262265 reports several media additions that enhance regenerability ofcallus of two inbred lines. Other published reports also indicated that“nontraditional” tissues are capable of producing somatic embryogenesisand plant regeneration. K. P. Rao, et al., Maize Genetics CooperationNewsletter, 60:64 65 (1986), refers to somatic embryogenesis from glumecallus cultures and B. V. Conger, et al., Plant Cell Reports, 6:345 347(1987) indicates somatic embryogenesis from the tissue cultures of maizeleaf segments. Thus, it is clear from the literature that the state ofthe art is such that these methods of obtaining plants are, and were,“conventional” in the sense that they are routinely used and have a veryhigh rate of success.

Tissue culture procedures of maize are described in Green and Rhodes,“Plant Regeneration in Tissue Culture of Maize,” Maize for BiologicalResearch (Plant Molecular Biology Association, Charlottesville, Va.1982, at 367 372) and in Duncan, et al., “The Production of CallusCapable of Plant Regeneration from Immature Embryos of Numerous Zea maysGenotypes,” 165 Planta 322 332 (1985). Thus, another aspect of thisinvention is to provide cells that upon growth and differentiationproduce maize plants having the physiological and morphologicalcharacteristics of the present invention. In an embodiment of theinvention, cells of the present invention are transformed genetically,for example with one or more genes described above, by using atransformation method described in U.S. Pat. No. 6,114,608, whereintransgenic plants of the present invention are obtained and used for theproduction of hybrid maize plants.

The introgression of a Bt11 event into a maize line, such as presentinvention, by backcrossing is exemplified in U.S. Pat. No. 6,114,608,and the present invention is directed to methods of introgressing a Bt11event into present invention and to progeny thereof using, for example,the markers described in U.S. Pat. No. 6,114,608.

Direct selection may be applied where the trait acts as a dominanttrait. An example of a dominant trait is herbicide tolerance. For thisselection process, the progeny of the initial cross are sprayed with theherbicide prior to the backcrossing. The spraying eliminates any plantthat does not have the desired herbicide tolerance characteristic, andonly those plants that have the herbicide tolerance gene are used in thesubsequent backcross. This process is then repeated for the additionalbackcross generations.

Maize is used as food, feed, and in industry. Sweet corn is canned andfrozen for human consumption. Maize, food products such as grits, meal,flour, starch, maize syrups, and dextrose also come from the dry- andwet-milling industries. Maize oil from maize germ is also a by-productof the milling industries.

Maize is a primary livestock feed for cattle, hogs, and poultry.Industrial use of maize includes production of ethanol, starch orpastes. The industrial applications of maize paste require specificviscosity, adhesiveness, and suspension characteristics. The paste andflour are used in paper and textiles.

The seed of the present invention or of the present invention furthercomprising one or more single gene traits, the plant produced from theinbred seed, the hybrid maize plant produced from the crossing of theinbred, hybrid seed, and various parts of the hybrid maize plant can beutilized for human food, livestock feed, and as a raw material inindustry.

The present invention therefore also discloses an agricultural productcomprising a plant of the present invention or derived from a plant ofthe present invention. The present invention also discloses anindustrial product comprising a plant of the present invention orderived from a plant of the present invention. The present inventionfurther discloses methods of producing an agricultural or industrialproduct comprising planting seeds of the present invention, growingplant from such seeds, harvesting the plants and processing them toobtain an agricultural or industrial product.

A deposit of at least 2500 seeds of this invention will be maintained bySyngenta Seed Inc. Access to this deposit will be available during thependency of this application to the Commissioner of Patents andTrademarks and persons determined by the Commissioner to be entitledthereto upon request. All restrictions on availability to the public ofsuch material will be removed upon issuance of a granted patent of thisapplication by depositing at least 2500 seeds of this invention at theAmerican Type Culture Collection (ATCC), at 10801 University Boulevard,Manassas, Va. 20110. The ATCC number of the deposit is PTA-10440. Thedate of deposit was Oct. 22, 2009 and the seed was tested on Nov. 2,2009 and found to be viable. The deposit of at least 2500 seeds will befrom inbred seed taken from the deposit maintained by Syngenta Seed Inc.The ATCC deposit will be maintained in that depository, which is apublic depository, for a period of 30 years, or 5 years after the lastrequest, or for the enforceable life of the patent, whichever is longer,and will be replaced if it becomes nonviable during that period.

Additional public information on patent variety protection may beavailable from the PVP Office, a division of the U.S. Government.

Accordingly, the present invention has been described with some degreeof particularity directed to the embodiment of the present invention. Itshould be appreciated, though that the present invention is defined bythe following claims construed in light of the prior art so thatmodifications or changes may be made to the embodiment of the presentinvention without departing from the inventive concepts containedherein.

1. A seed of the maize inbred line G07-NPID5459, representative seed ofsaid line having been deposited under ATCC Accession Number PTA-10440.2. A maize plant or plant part produced by growing the seed of claim 1.3. An F1 hybrid maize seed produced by crossing a plant of maize inbredline G07-NPID5459 according to claim 2 with a different maize plant andharvesting the resultant F1 hybrid maize seed.
 4. A maize plant or plantpart produced by growing the F1 hybrid maize seed of claim
 3. 5. An F1hybrid maize seed comprising an inbred maize plant cell of inbred maizeline G07-NPID5459, representative seed of said line having beendeposited under ATCC Accession Number PTA-10440.
 6. A maize plantproduced by growing the F1 hybrid maize seed of claim
 5. 7. A cell of amaize plant produced by growing the F1 hybrid maize seed of claim
 5. 8.A process of introducing a desired trait into maize inbred lineG07-NPID5459 comprising: (a) crossing G07-NPID5459 plants grown fromG07-NPID5459 seed, representative seed of which has been deposited underATCC Accession Number PTA-10440, with plants of another maize line thatcomprise a desired trait to produce F1 progeny plants, wherein thedesired trait is selected from the group consisting of waxy starch, malesterility, herbicide resistance, insect resistance, bacterial diseaseresistance, fungal disease resistance, and viral disease resistance; (b)selecting F1 progeny plants that have the desired trait to produceselected F1 progeny plants; (c) crossing the selected progeny plantswith the G07-NPID5459 plants to produce backcross progeny plants; (d)selecting for backcross progeny plants that have the desired trait toproduce selected backcross progeny plants; and (e) repeating steps (c)and (d) at least three or more times to produce backcross progeny plantsthat comprise the desired trait and all of the physiological andmorphological characteristics of corn inbred line G07-NPID5459 listed inTable 1 when grown in the same environmental conditions.
 9. A plantproduced by the process of claim
 8. 10. A maize plant having all thephysiological and morphological characteristics of inbred lineG07-NPID5459, wherein a sample of the seed of inbred line G07-NPID5459was deposited under ATCC Accession Number PTA-10440.
 11. A process ofproducing maize seed, comprising crossing a first parent maize plantwith a second parent maize plant, wherein one or both of the first orthe second parent maize plants is the plant of claim 10, and harvestingthe resultant seed.
 12. The maize seed produced by the process of claim11.
 13. The maize seed of claim 12, wherein the maize seed is hybridseed.
 14. A hybrid maize plant, or its parts, produced by growing saidhybrid seed of claim
 13. 15. The maize plant of claim 10, furthercomprising a genome comprising at least one transgene or a geneconversion conferred by a transgene.
 16. The maize plant of claim 15,wherein the gene confers a trait selected from the group consisting ofherbicide tolerance; insect tolerance; resistance to bacterial, fungal,nematode or viral disease; waxy starch; male sterility or restoration ofmale fertility, modified carbohydrate metabolism and modified fatty acidmetabolism.
 17. A method of producing a maize plant derived from theinbred line G07-NPID5459, the method comprising the steps of (a) growinga progeny plant produced by crossing the plant of claim 10 with a secondmaize plant; (b) crossing the progeny plant with itself or a differentplant to produce a seed of a progeny plant of a subsequent generation;(c) growing a progeny plant of a subsequent generation from said seedand crossing the progeny plant of a subsequent generation with itself ora different plant; and (d) repeating steps (b) and (c) for an additional0-5 generations to produce a maize plant derived from the inbred lineG07-NPID5459.
 18. A method for developing a maize plant in a maize plantbreeding program, comprising applying plant breeding techniques to themaize plant of claim 10, or its parts, wherein application of saidtechniques results in development of a maize plant.
 19. The method fordeveloping a maize plant in a maize plant breeding program of claim 18,wherein the plant breeding techniques are selected from the groupconsisting of recurrent selection, backcrossing, pedigree breeding,restriction fragment length polymorphism enhanced selection, geneticmarker enhanced selection, and transformation.